By Prof. Dr. Christian Rittner, Dr. rer. nat. Peter M. Schneider (auth.), Prof. Dr. Christian Rittner, Dr. rer. nat. Peter M. Schneider (eds.)
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Extra info for Advances in Forensic Haemogenetics: 14th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft for forensische Hämogenetik e.V.), Mainz, September 18–21, 1991
The fragments in Iane 8 demonstrate the problems with the gels presently in use. The correct assignment of unknown alleles in such gels is impossible, although they give a high resolution for the human standard REFERENCES Baird M, Balazc 1, Giusti A, Miyazaki L, Nicholas L, Wexler K, Kanter E, Glassberg J, Allen F, Rubinstein, P. and Sussman, L. (1986) Allele frequency distribution to two highly polymorphic DNA sequences in three ethnic groups and its application to the determination of paternity.
1990), therefore fragments recovered by Priestley et al. are 72 bp longer than those of Wu et al.. Priestley et al. described 5 alleles, in the range between 650 aud 890 bp while Wu et al. described 4 alleles in the range 600 - 700 bp. The repeat sequence can be 31 or 34 bp long (Stoker et al. 1985) aud is inherited in a mendelian fashion. This situation can therefore lead to fragments which are different in composition but identica) in their electrophoretic mobility. In spite of these considerations the aim of this investigation was to construct a human DNA standard to achieve better comparability.
Schneider © Springer·Verlag Berlin Heidelberg 1992 36 that moves back and forth in a direction perpendicular to the direction of electrophoresis, permitting the analysis of 24 lanes per gel. A filter wheel is placed in the optica! pathway and made to rotate in synchrony with the scanning stage to permit the discrimination of the fluorescence emission bands of multiple dyes. The filtered emitted light is collected by a photomultiplyer and converted to digital data. Analysis software can correct for the spectral overlap of the blue, green, yellow, and red fluorescence emitted by the dyes and yields a collection file, from which one obtains a presentation of the data as a familiar gel image.