Press "Enter" to skip to content

Agrobacterium Protocols, Second Edition: Volume II (Methods by Kan Wang

By Kan Wang

Show description

Read or Download Agrobacterium Protocols, Second Edition: Volume II (Methods in Molecular Biology Vol 344) PDF

Best biology books

CliffsAP 5 Biology Practice Exams (Cliffs AP)

The entire advisor to a better ranking at the *AP Biology examination

Why CliffsAP Guides?

choose the identify you recognize and belief

Get the data you need--fast!

Written by means of test-prep experts

in regards to the contents:

* Describes the exam's structure
* supplies confirmed suggestions for answering multiple-choice and free-response questions

five Full-length AP Biology perform tests
* provide the perform and self belief you must prevail
* dependent just like the real examination so that you recognize what to anticipate and discover ways to allot time accurately

* each one perform examination includes:
* Multiple-choice questions
* Free-response questions
* a solution key plus specified factors
* A consultant to scoring the perform examination

*AP is a registered trademark of the varsity Board, which used to be no longer focused on the construction of, and doesn't propose, this product.

AP Test-Prep necessities from the specialists at CliffsNotes?

A New Biology for the 21st Century

Now greater than ever, biology has the capability to give a contribution useful suggestions to a number of the significant demanding situations confronting the us and the area. a brand new Biology for the twenty first Century recommends "New Biology" approach--one that is dependent upon higher integration inside biology, and nearer collaboration with actual, computational, and earth scientists, mathematicians and engineers--be used to discover recommendations to 4 key societal wishes: sustainable foodstuff construction, environment recovery, optimized biofuel creation, and development in human healthiness.

Protocells - Bridging Nonliving and Living Matter

Protocells bargains a finished source on present makes an attempt to create uncomplicated sorts of existence from scratch within the laboratory. those minimum types of cells, often called protocells, are entities with sensible homes produced from nonliving fabrics, and the publication offers in-depth investigations of approaches on the interface among nonliving and residing subject.

Dictyostelium discoideum Protocols (Methods in Molecular Biology Vol 346)

Dictyostelium discoideum Protocols offers a entire number of state of the art molecular organic protocols for learning D. discoideum, with emphasis on its software within the learn of basic mobile tactics together with sign transduction, chemotaxis, mobile motility, cytokinesis, phagocytosis, and points of improvement resembling cellphone sorting, development formation, and mobile kind differentiation.

Additional info for Agrobacterium Protocols, Second Edition: Volume II (Methods in Molecular Biology Vol 344)

Example text

The stem pieces are then rinsed three times with sterilized distilled water and blotdried on sterile No. 1 Whatman filter paper. 5. The stem pieces are cultured by inserting the basal stem portion into Magenta boxes containing 50 mL semi-solid MS shoot multiplication medium (4E). 6. Cultures are incubated at 26°C under a 12-h light/dark regime provided by coolwhite fluorescent lamps (40 µmole/m2/s) until young actively growing apical leaves emerge (about 3 wk after culture). 7. ). The in vitro plants can supply apical leaves for up to 3 mo.

The culture is transferred to a sterile tube and spun at 700–1000g for 15 mins and then pellet resuspended in 15 mL of MS20. 6. At this stage, 5 mL of the culture can be taken and used to make 1 mL glycerol stocks, 650 µL of culture medium to 350 µL of 50% Glycerol (v:v) and the samples flash frozen and stored at −80°C. 3. Inoculation, Co-Cultivation, and Regeneration 1. 5-mm wide excised from 3- to 4-wk old potato stock plants (see Note 9). 2. Explants (up to 30 per plate) are placed into Petri dishes containing 15 mL of MS20 to avoid dessication (see Note 10).

Are transferred to embryo maturation medium (RM1) and incubated at 26oC under a 12-hr light/dark regime. 2. 05% (v/v) Tween-20. 3. The germinated somatic embryos are then cut into pieces approxi 25–50 mm2 and are wounded using a sterile 38-mm long BD1M needle prior to inoculation with Agrobacterium (see Fig. 1). 4. Preparation of Auxiliary Buds 1. 1. 2. Buds are excised using a No 10 surgical blade. A small incision is made on the surface of the excised bud. 20 Msikita et al. Fig. 2. Overall scheme of Agrobacterium-mediated transformation of cassava.

Download PDF sample

Rated 4.60 of 5 – based on 36 votes