By Afaf Kamal-Eldin, Jan Pokorny
Lipid oxidation, even though researched because the starting of the 20 th century, nonetheless provides no whole and passable info at the composition of oxidized lipids. One very important issue contributing to those gaps in our wisdom approximately lipid oxidation pertains to the shortages in analytical technique. Analytical equipment compatible for oxidized lipids have been usually reviewed within the final decade, yet in most cases from the element of choice of person oxidized lipid periods, comparable to peroxides, aldehydes, polar lipids, or polymers. during this e-book, they're taken care of from the perspective of sorts of analytical equipment used, together with diverse volumetric equipment, UV-visible spectrometric equipment, excessive functionality size-exclusion chromatography, nuclear magnetic resonance spectroscopy, electron spin resonance spectroscopy, and differential scanning calorimetry. research of Lipid Oxidation is key for additional advancements in analytical method and hyphenated strategies, with which extra knowing of the response kinetics, mechanism, and implications will ensue.
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Extra info for Analysis of lipid oxidation
Nonpolar and polar fractions are evaporated under reduced pressure, and separation is verified by thin-layer chromatography (TLC) (Fig. 2). After gravimetric determination of polar compounds, the polar fractions are further analyzed by HPSEC, using two columns (100 and 500 Å), packed with a porous, highly cross-linked styrene divinylbenzene copolymer (particle size: 5 µm), connected in series. THF serves as the mobile phase, at a flow rate of 1 mL/min and a refractive index detector is used. HPSEC analysis requires a total run time of only 15 min.
Combination of Adsorption Chromatography and HPSEC Silica Column and HPSEC. In 1988, the first methodology based on the combination of adsorption chromatography using silica columns and HPSEC for quantita- Copyright © 2005 AOCS Press Ch4(OxiAnalysis)(40-69)Co1 3/23/05 8:49 PM Page 43 Fig. 1. Schematic representation of methodologies based on HPSEC for quantitation of nonvolatile oxidation compounds. Abbreviations: TG, triacylglycerols; FAME, fatty acid methyl esters. Copyright © 2005 AOCS Press Ch4(OxiAnalysis)(40-69)Co1 3/23/05 8:49 PM Page 44 tion of oxidized and hydrolytic compounds was published (Dobarganes et al.
And Täufel, K. (1964) Analysis of Carbonyl Compounds in the Presence of Hydroperoxides After Reduction with Potassium Iodide, Fette Seifen Anstrichm. 66, 866–869 and 1052–1058. Løvaas, E. (1992) A Sensitive Spectrophotometric Method for Lipid Hydroperoxide Determination, J. Am. Oil Chem. Soc. 69, 777–783. Marcuse, R. (1970) Studien über den TBS Test, 1. Seine Temperaturabhängigkeit und Anwendung auf Vakuum-destillierte Fettoxydationsprodukte, Fette Seifen Anstrichm. 72, 635–640. , and Pokorn´y , J.