By Mitchelson, Cheng
A great panel of hands-on specialists and builders of CE gear describe in step by step style their top state of the art tools for the detection and research of DNA mutations and transformations, starting from certain DNA loci to whole genomes of organisms. This moment quantity of the set, useful functions of Capillary Electrophoresis, covers thoughts for high-throughput research of DNA fragments utilizing SNP detection, mutation detection, DNA sequencing tools, and DNA-ligand interactions. numerous chapters additionally talk about CE research at increased temperatures and using micro-CE and array-CE units. The spouse quantity, creation to the Capillar Electrophoresis of Nucliec Acids, deals quite simply reproducible tools for the research of small oligonucleotides and transformed nucleotides, and time-tested suggestion on instrumentation, sign detection, the capillary setting, and the mixing of mass spectrometry with CE. complete and up to date, the paired volumes of Capillary Electrophoresis of Nucleic Acids provide an authoritative consultant with quick access to quickly, flexible, trustworthy, and robust applied sciences for all these uncomplicated and scientific investigators studying DNA version this present day.
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Additional info for Capillary Electrophoresis of Nucleic Acids Volume 2 Practical Applications of Capillary Electrophoresis (Methods in Molecular Biology Vol 163)
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The detection of all lanes with high temporal resolution is achieved by using a laserexcited confocal fluorescence scanner. Figure 12 shows the microdevice used for electrophoretic separation with fluorescence detection for the diagnosis of hereditary hemochromatosis. Electropherograms represent two sequential separations of RsaIdigested HFE amplicons. The three genotypes are characterized by a single peak at 140 bp corresponding to the 845G type, a single peak at 111 bp corresponding to the 845A type, and the heterozygote type that exhibits both the 140- and 111-bp peaks.
11. 4. 12. SYBR Green II dye. 13. Fluorescein at a concentration of 10–8 M. 14. 8. 2. Chip Electrophoresis 1. Electrophoresis chip with integrated electrochemical detection. 2. 8 with 1 µM EtBr. 3. DNA: RsaI-digested HFE amplicons. 4. Standard DNA: ΦX174/HaeIII digest. 5. Salmonella PCR product. 3. 1. Examples of Fast DNA Separations 1. This section illustrates the potential of CE for ultra-fast separations. CE decreases the analysis time typically by ten times compared to standard slab-gel electrophoresis.