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Nucleic Acids and Molecular Biology by M. Sip, M. Leng (auth.), Prof. Dr. Fritz Eckstein, Prof. Dr.

By M. Sip, M. Leng (auth.), Prof. Dr. Fritz Eckstein, Prof. Dr. David M. J. Lilley (eds.)

Molecular biology is without doubt one of the such a lot speedily transforming into develo- ping and at thesame time most enjoyable disciplines. the major to molecular biology lies within the knowing of nucleic a- cids - their constitution, functionality, and interplay with seasoned- teins. Nucleic Acids and Molecular Biology retains scientists proficient of the explosively starting to be info and complies with with the nice curiosity during this box via delivering a persevered excessive ordinary of overview. a considerable a part of this quantity has been dedicated to the research of alternative elements of nucleic acid-protein-interactions together with RNA- protein-interaction.

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Sample text

This complicates the analysis of binding specificity in such an assay, as it is partly dependent on the polylinker sequence. The difficulty was overcome by the design of the met "antibox" (CCGGCAGG), an 8-bp sequence where each position is occupied by the least frequently occurring base in the natural met box sequences, as opposed to the most frequent which defines the consensus met box. This would be expected to be a poor binding site for met repressor, and, indeed, when the consensus two met box operator insert is flanked by two antiboxes in the assay fragment (Fig.

The only direct contacts to the base pairs are made by two side chains from each strand of the f1-ribbon. e. GlO of the bottom strand. There are, therefore, eight direct hydrogen bonds to bases in the complex, when all the symmetry-related interactions are taken into account (Fig. 6). The f1-ribbon makes rather a loose fit in the major groove as judged by the van der Waals surfaces, leaving room for a number of solvent molecules to intrude. Only four of these are sufficiently well ordered to be visible in electron density maps at this resolution.

Phosphate groups showing strong ethylation interference are marked with (*). Contacts to the repressor are shown schematically, with direct base contacts above the line, and contacts to phosphates below. e. to the bottom strand for base contacts and the top strand for phosphate contacts, while non-italicised labels indicate base contacts to the top strand and phosphate contacts to the bottom strand. 66 and no significant bending. The DNA is bent, however, towards each repressor at the centre of each met box.

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