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The Molecular Biology of Insect Disease Vectors: A Methods by Mark Q. Benedict (auth.), Julian M. Crampton, C. Ben Beard,

By Mark Q. Benedict (auth.), Julian M. Crampton, C. Ben Beard, Christos Louis (eds.)

Only one new release in the past, entomology used to be a proudly remoted self-discipline. In Comstock corridor, the development of the dept of Entomology at Cornell collage the place i used to be first brought to experimental technological know-how within the laboratory of Tom Eisner, these folks drawn to the chemistry of existence felt like interlopers. within the 35 years that experience elapsed when you consider that then, all of biology has replaced, and entomology with it. smug molecular biologists and green with envy classical biologists may well imagine that what has occurred is a adverse take-over of biology via molecular biology. yet they're improper. increasingly more we now remember the fact that the occasions have been happier and masses extra interesting, amounting to a brand new synthesis. Molecular Biology, which used to be at the beginning fascinated about the best of organisms, micro organism and viruses, broke out of its confines after the preliminary primary questions have been responded - the constitution of DNA, the genetic code, the character of regulatory genes - and, importantly, as its equipment grew to become progressively more as a rule appropriate. The recombinant DNA revo­ lution of the Seventies, the improvement of strategies for sequencing macromolecules, the polymerase chain response, new molecular equipment of genetic research, all introduced molecular biology nose to nose with the countless complexity and the exuber­ ant variety of lifestyles. Molecular biology itself stopped being an remoted self-discipline, pre­ fascinated by the common legislation of existence, and have become an method of addressing fas­ cinating particular difficulties from each box of biology.

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Additional resources for The Molecular Biology of Insect Disease Vectors: A Methods Manual

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20, 568-569. , Ferro De Carrasquilla, C. , (1984) Establecimiento de una colonia de Lutzomyia walkeri (Newstead, 1914) (Diptera: Phlebotominae). Biomedica 4, 37-41. D. (1977) The colonization of Lutzomyiaflaviscutellata (Diptera: Psychodidae), a vector of Leishmania mexicana amazonensis in Brazil. J. Med. Entomol. 14,469-476. , Lainson, R. J. (1978) Some methods for membrane feeding of laboratory reared, neotropical sandffies (Diptera: Psychodidae). Ann. Trop. Med. Parasit. 72, 269-276. V. G. (1981) A larval diet for rearing phlebotomine sandffies (Diptera: Psychodidae).

Med. Entomol. 14,469-476. , Lainson, R. J. (1978) Some methods for membrane feeding of laboratory reared, neotropical sandffies (Diptera: Psychodidae). Ann. Trop. Med. Parasit. 72, 269-276. V. G. (1981) A larval diet for rearing phlebotomine sandffies (Diptera: Psychodidae). J. Med. Entomol. 18, 446. CHAPTER FOUR Care and maintenance of blackfly colonies Eddie W Cupp and Frank B. 1 INTRODUCTION The availability of vector species on a continuous basis in the laboratory is an important prerequisite for both basic and applied aspects of research.

2 MATERIALS Rearing'colonizing simuliid species under ordinary laboratory circumstances differs fundamentally from other vector arthropods in that a reliable closed system of water recirculation is required to promote development of the immature stages. Because of this peculiarity and the lack of commercially available equipment for this task, difMolecular Biology of Insect DisetlSe Vectors: A methods manual. M. B. Beard and C. Louis. Published in 1997 by Chapman & HaIl. ISBN 041273660 8. 32 Care and Maintenance of Insect Colonies ferent types of specialized rearing devices and ancillary equipment have been handmade and used successfully over the past several decades for this purpose.

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